Twitter
|
Facebook
|
LinkedIn
|
Zhihu
|
Weibo
Year
Month
(Peer-Reviewed) 3D imaging lipidometry in single cell by in-flow holographic tomography
Daniele Pirone ¹ ², Daniele Sirico ¹ ³, Lisa Miccio ¹, Vittorio Bianco ¹, Martina Mugnano ¹, Danila del Giudice ¹ ⁴, Gianandrea Pasquinelli ⁵ ⁶, Sabrina Valente ⁵, Silvia Lemma ⁷ ⁸ ⁹, Luisa Iommarini ⁹ ¹⁰, Ivana Kurelac ⁷ ⁸ ⁹, Pasquale Memmolo ¹, Pietro Ferraro ¹
¹ CNR-ISASI, Institute of Applied Sciences and Intelligent Systems “E. Caianiello”, Via Campi Flegrei 34, Pozzuoli, Napoli 80078, Italy
² Department of Electrical Engineering and Information Technologies, University of Naples “Federico II”, via Claudio 21, Napoli 80125, Italy
³ Department of Chemical, Materials and Production Engineering of the University of Naples Federico II, Piazzale Tecchio 80, Napoli 80125, Italy
⁴ Department of Mathematics and Physics, University of Campania “Luigi Vanvitelli”, Caserta 81100, Italy
⁵ Biotechnology and Methods in Laboratory Medicine, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna 40126, Italy
⁶ Subcellular Nephro-Vascular Diagnostic Program, Pathology Unit S.Orsola IRCCS, University of Bologna, Bologna 40126, Italy
⁷ Unit of Medical Genetics, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Via Massarenti 9, Bologna 40138, Italy
⁸ Study and Research Center on Gynecological Neoplasias, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Via Massarenti 9, Bologna 40138, Italy
⁹ Center for Applied Biomedical Research (CRBA), University of Bologna, Bologna 40138, Italy
¹⁰ Department of Pharmacy and Biotechnology (FABIT), University of Bologna, Bologna 40126, Italy
Opto-Electronic Advances, 2022-08-25
https://doi.org/10.29026/oea.2023.220048
https://www.oejournal.org/article/doi/10.29026/oea.2023.220048
Abstract

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets (LDs) in several regulatory mechanisms in living cells. LDs are dynamic organelles and therefore their complete characterization in terms of number, size, spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs.

Until now, fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity. However, such methods generally only provide 2D assays and partial measurements. Furthermore, both can be destructive and with low productivity, thus limiting analysis of large cell numbers in a sample.

Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer, by using ovarian cancer cells and monocyte cell lines as models. A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported.

The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.
3D imaging lipidometry in single cell by in-flow holographic tomography_1
3D imaging lipidometry in single cell by in-flow holographic tomography_2
3D imaging lipidometry in single cell by in-flow holographic tomography_3
3D imaging lipidometry in single cell by in-flow holographic tomography_4
  • The possibilities of using a mixture of PDMS and phosphor in a wide range of industry applications
  • Rodrigo Rendeiro, Jan Jargus, Jan Nedoma, Radek Martinek, Carlos Marques
  • Opto-Electronic Advances
  • 2024-09-20
  • Agile cavity ringdown spectroscopy enabled by moderate optical feedback to a quantum cascade laser
  • Qinxue Nie, Yibo Peng, Qiheng Chen, Ningwu Liu, Zhen Wang, Cheng Wang, Wei Ren
  • Opto-Electronic Advances
  • 2024-09-20
  • Genetic algorithm assisted meta-atom design for high-performance metasurface optics
  • Zhenjie Yu, Moxin Li, Zhenyu Xing, Hao Gao, Zeyang Liu, Shiliang Pu, Hui Mao, Hong Cai, Qiang Ma, Wenqi Ren, Jiang Zhu, Cheng Zhang
  • Opto-Electronic Science
  • 2024-09-20
  • Finely regulated luminescent Ag-In-Ga-S quantum dots with green-red dual emission toward white light-emitting diodes
  • Zhi Wu, Leimeng Xu, Jindi Wang, Jizhong Song
  • Opto-Electronic Advances
  • 2024-09-18
  • Vortex-field enhancement through high-threshold geometric metasurface
  • Qingsong Wang, Yao Fang, Yu Meng, Han Hao, Xiong Li, Mingbo Pu, Xiaoliang Ma, Xiangang Luo
  • Opto-Electronic Advances
  • 2024-09-10
  • Cascaded metasurfaces enabling adaptive aberration corrections for focus scanning
  • Xiaotong Li, Xiaodong Cai, Chang Liu, Yeseul Kim, Trevon Badloe, Huanhuan Liu, Junsuk Rho, Shiyi Xiao
  • Opto-Electronic Advances
  • 2024-09-06
  • Functionality multiplexing in high-efficiency metasurfaces based on coherent wave interferences
  • Yuejiao Zhou, Tong Liu, Changhong Dai, Dongyi Wang, Lei Zhou
  • Opto-Electronic Advances
  • 2024-09-03
  • Physics and applications of terahertz metagratings
  • Shreeya Rane, Shriganesh Prabhu, Dibakar Roy Chowdhury
  • Opto-Electronic Science
  • 2024-09-03
  • Surface-patterned chalcogenide glasses with high-aspect-ratio microstructures for long-wave infrared metalenses
  • Zhaofeng Gu, Yixiao Gao, Kongsi Zhou, Junyang Ge, Chen Xu, Lei Xu, Mohsen Rahmani, Ran Jiang, Yimin Chen, Zijun Liu, Chenjie Gu, Yaoguang Ma, Jianrong Qiu, Xiang Shen
  • Opto-Electronic Science
  • 2024-09-03
  • Racemic dielectric metasurfaces for arbitrary terahertz polarization rotation and wavefront manipulation
  • Jie Li, Xueguang Lu, Hui Li, Chunyu Song, Qi Tan, Yu He, Jingyu Liu, Li Luo, Tingting Tang, Tingting Liu, Hang Xu, Shuyuan Xiao, Wanxia Huang, Yun Shen, Yan Zhang, Yating Zhang, Jianquan Yao
  • Opto-Electronic Advances
  • 2024-08-28
  • Miniature meta-device for dynamic control of Airy beam
  • Qichang Ma, Guixin Li
  • Opto-Electronic Advances
  • 2024-08-28
  • Multi-prior physics-enhanced neural network enables pixel super-resolution and twin-image-free phase retrieval from single-shot hologram
  • Xuan Tian, Runze Li, Tong Peng, Yuge Xue, Junwei Min, Xing Li, Chen Bai, Baoli Yao
  • Opto-Electronic Advances
  • 2024-08-28



    • Learning-based joint UAV trajectory and power allocation optimization for secure IoT networks                                Carnivorous plants inspired shape-morphing slippery surfaces
    About
    |
    Contact
    |
    Copyright © PubCard