Year
Month
(Peer-Reviewed) SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy
Xingyu Li 李兴宇 ¹, Peili Hou 侯佩莉 ¹, Wenqing Ma 马文青 ¹, Xuefeng Wang 王雪峰 ², Hongmei Wang 王洪梅 ¹, Zhangping Yu ¹, Huasong Chang ¹, Tiecheng Wang 王铁成 ², Song Jin ¹, Xue Wang ¹, Wenqi Wang ¹ ², Yudong Zhao ², Yong Zhao ², Chunqing Xu ¹, Xiaomei Ma ¹, Yuwei Gao 高玉伟 ², Hongbin He 何洪彬 ¹
¹ Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, 250014, People’s Republic of China
中国 济南 山东师范大学生命科学学院 反刍动物疾病研究中心
² Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, People’s Republic of China
中国 长春 中国农业科学院长春兽医研究所
Abstract

The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused severe morbidity and mortality in humans. It is urgent to understand the function of viral genes. However, the function of open reading frame 10 (ORF10), which is uniquely expressed by SARS-CoV-2, remains unclear.

In this study, we showed that overexpression of ORF10 markedly suppressed the expression of type I interferon (IFN-I) genes and IFN-stimulated genes. Then, mitochondrial antiviral signaling protein (MAVS) was identified as the target via which ORF10 suppresses the IFN-I signaling pathway, and MAVS was found to be degraded through the ORF10-induced autophagy pathway. Furthermore, overexpression of ORF10 promoted the accumulation of LC3 in mitochondria and induced mitophagy. Mechanistically, ORF10 was translocated to mitochondria by interacting with the mitophagy receptor Nip3-like protein X (NIX) and induced mitophagy through its interaction with both NIX and LC3B. Moreover, knockdown of NIX expression blocked mitophagy activation, MAVS degradation, and IFN-I signaling pathway inhibition by ORF10.

Consistent with our observations, in the context of SARS-CoV-2 infection, ORF10 inhibited MAVS expression and facilitated viral replication. In brief, our results reveal a novel mechanism by which SARS-CoV-2 inhibits the innate immune response; that is, ORF10 induces mitophagy-mediated MAVS degradation by binding to NIX.
SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy_1
SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy_2
SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy_3
SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy_4
  • High-resolution tumor marker detection based on microwave photonics demodulated dual wavelength fiber laser sensor
  • Jie Hu, Weihao Lin, Liyang Shao, Chenlong Xue, Fang Zhao, Dongrui Xiao, Yang Ran, Yue Meng, Panpan He, Zhiguang Yu, Jinna Chen, Perry Ping Shum
  • Opto-Electronic Advances
  • 2024-12-16
  • High performance laser induced plasma assisted ablation by GHz burst mode femtosecond pulses
  • Jingbo Yin, Yulong Zhao, Minghui Hong
  • Opto-Electronic Advances
  • 2024-12-16
  • Sequential harmonic spin–orbit angular momentum generation in nonlinear optical crystals
  • Yutao Tang, Zixian Hu, Junhong Deng, Kingfai Li, Guixin Li
  • Opto-Electronic Advances
  • 2024-12-16
  • Design, setup, and facilitation of the speckle structured illumination endoscopic system
  • Elizabeth Abraham, Zhaowei Liu
  • Opto-Electronic Science
  • 2024-12-13
  • Ultra-high-Q photonic crystal nanobeam cavity for etchless lithium niobate on insulator (LNOI) platform
  • Zhi Jiang, Cizhe Fang, Xu Ran, Yu Gao, Ruiqing Wang, Jianguo Wang, Danyang Yao, Xuetao Gan, Yan Liu, Yue Hao, Genquan Han
  • Opto-Electronic Advances
  • 2024-10-31
  • Advanced biological imaging techniques based on metasurfaces
  • Yongjae Jo, Hyemi Park, Hyeyoung Yoon, Inki Kim
  • Opto-Electronic Advances
  • 2024-10-31
  • Orthogonal matrix of polarization combinations: concept and application to multichannel holographic recording
  • Shujun Zheng, Jiaren Tan, Hongjie Liu, Xiao Lin, Yusuke Saita, Takanori Nomura, Xiaodi Tan
  • Opto-Electronic Advances
  • 2024-10-23
  • Streamlined photonic reservoir computer with augmented memory capabilities
  • Changdi Zhou, Yu Huang, Yigong Yang, Deyu Cai, Pei Zhou, Kuenyao Lau, Nianqiang Li, Xiaofeng Li
  • Opto-Electronic Advances
  • 2024-10-22
  • High-precision multi-focus laser sculpting of microstructured glass
  • Kang Xu, Peilin Huang, Lingyu Huang, Li Yao, Zongyao Li, Jiantao Chen, Li Zhang, Shaolin Xu
  • Opto-Electronic Advances
  • 2024-10-09
  • Multi-physical field null medium: new solutions for the simultaneous control of EM waves and heat flow
  • Sailing He, Ruili Zhang, Junbo Liang
  • Opto-Electronic Advances
  • 2024-09-30
  • Adaptive decentralized AI scheme for signal recognition of distributed sensor systems
  • Shixiong Zhang, Hao Li, Cunzheng Fan, Zhichao Zeng, Chao Xiong, Jie Wu, Zhijun Yan, Deming Liu, Qizhen Sun
  • Opto-Electronic Advances
  • 2024-09-29
  • Data-driven polarimetric approaches fuel computational imaging expansion
  • Sylvain Gigan
  • Opto-Electronic Advances
  • 2024-09-28



  • The vicK gene of Streptococcus mutans mediates its cariogenicity via exopolysaccharides metabolism                                Computational Assessment of the Expression-modulating Potential for Noncoding Variants
    About
    |
    Contact
    |
    Copyright © PubCard