(Peer-Reviewed) Label-free trace detection of bio-molecules by liquid-interface assisted surface-enhanced Raman scattering using a microfluidic chip
Shi Bai ¹, Xueli Ren ², Kotaro Obata ¹, Yoshihiro Ito ², Koji Sugioka ¹
¹ Advanced Laser Processing Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
² Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Opto-Electronic Advances, 2022-08-20
Abstract
Surface-enhanced Raman scattering (SERS), owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals, is recently attracting much attention to be used for biotechnology, such as cell imaging and tumor therapy. On the other hand, the trace detection of bio-molecules with large molecular weight is still challenging because the troublesome treatment of SERS substrate using coupling or cross-linking agents is required.
In this paper, we apply liquid interface assisted SERS (LI-SERS) method, which provides unique features of collection and self-immobilization of analyte molecules on the SERS substrate, to realize the label-free trace detection of bio-molecules with detection limits of pM ~ fM. Specifically, deoxyribonucleic acid (DNA) discrimination and quantitative detection of β-Amyloid (Aβ) in trace-concentration are demonstrated to illustrate the ultrahigh sensitivity and versatility of the LI-SERS method.
The results suggest LI-SERS is promising for the early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.
High-resolution tumor marker detection based on microwave photonics demodulated dual wavelength fiber laser sensor
Jie Hu, Weihao Lin, Liyang Shao, Chenlong Xue, Fang Zhao, Dongrui Xiao, Yang Ran, Yue Meng, Panpan He, Zhiguang Yu, Jinna Chen, Perry Ping Shum
Opto-Electronic Advances
2024-12-16
Ultra-high-Q photonic crystal nanobeam cavity for etchless lithium niobate on insulator (LNOI) platform
Zhi Jiang, Cizhe Fang, Xu Ran, Yu Gao, Ruiqing Wang, Jianguo Wang, Danyang Yao, Xuetao Gan, Yan Liu, Yue Hao, Genquan Han
Opto-Electronic Advances
2024-10-31