PubCard - Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex

(Peer-Reviewed) Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex

Cong Chen 陈聪 ¹ ², Ming Tan 谭明 ¹ ², Zhenfang Wu 吴振芳 ¹ ², Yuebin Zhang 张跃斌 ³, Fanyang He 贺钒洋 ¹ ², Yanjia Lu 鲁彦伽 ¹ ², Shaobai Li 李少白 ¹ ², Mi Cao 曹密 ¹ ², Guohui Li 李国辉 ³, Jian Wu 武健 ¹ ², Hong Cheng 程红 ⁴, Ming Lei 雷鸣 ¹ ² ⁵

¹ State Key Laboratory of Oncogenes and Related Genes, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China 上海交通大学医学院附属第九人民医院癌基因及相关基因国家重点实验室
² Shanghai Institute of Precision Medicine, Shanghai 200125, China 上海精准医学研究院
³ Laboratory of Molecular Modeling and Design, State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China 中国科学院大连化学物理研究所分子反应动力学国家重点实验室分子模拟与设计研究组
⁴ State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China 中国科学院上海生物化学与细胞生物学研究所中国科学院分子细胞科学卓越创新中心分子生物学国家重点实验室
⁵ Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 上海交通大学医学院细胞分化与凋亡教育部重点实验室

Science Bulletin, 2021-08-05

https://doi.org/10.1016/j.scib.2021.08.004

https://www.sciencedirect.com/science/article/abs/pii/S2095927321005545

Graphical abstract

During eukaryotic transcription, nascent mRNAs hybrid with the DNA template to form an R-loop structure. The THO-Sub2 complex is recruited and associates with the transcription machinery to unwind this harmful R-loop structure and ensure efficient mRNA export.