Year
Month
(Peer-Reviewed) Local renin angiotensin system and sperm DNA fragmentation
María Victoria Aparicio Prieto ¹, María Victoria Rodríguez Gallego ², Asier Valdivia Palacín ³, Yosu Franco Iriarte ⁴, Gotzone Hervás Barbara ³, Enrique Echevarría Orella ³, Luis Casis Saenz ³
¹ Human Reproduction Unit, Cruces University Hospital, Barakaldo 48903, Spain
² Human Reproduction Unit, San Pedro Hospital, Logroño 26006, Spain
³ Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Leioa 48940, Spain
⁴ Human Reproduction Unit, Ruber International Hospital, Madrid 28034, Spain
Asian Journal of Andrology, 2021-09-07
Abstract

The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. In this work, we analyzed the relationship between the RAS and DNA integrity. Fifty male volunteers were divided into two groups (25 each): control (DNA fragmentation ≤20%) and pathological (DNA fragmentation >20%) cases.

Activities of five peptidases controlling RAS were measured fluorometrically: prolyl endopeptidase (which converts angiotensin [A] I and A II to A 1–7), neutral endopeptidase (NEP/CD10: A I to A 1–7), aminopeptidase N (APN/CD13: A III to A IV), aminopeptidase A (A II to A III) and aminopeptidase B (A III to A IV). Angiotensin-converting enzyme (A I to A II), APN/CD13 and NEP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays, as were the receptors of all RAS components: A II receptor type 1 (AT1R), A II receptor type 2 (AT2R), A IV receptor (AT4R or insulin-regulated aminopeptidase [IRAP]), (pro)renin receptor (PRR) and A 1–7 receptor or Mas receptor (MasR) None of the enzymes that regulate levels of RAS components, except for APN/CD13 (decrease in fragmented cells), showed significant differences between both groups.

Micrographs of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented cells. Labeling of AT1R (94.3% normozoospermic vs 84.1% fragmented), AT4R (96.2% vs 95.3%) and MasR (97.4% vs 87.2%) was similar between the groups. AT2R (87.4% normozoospermic vs 63.1% fragmented) and PRR (96.4% vs 48.2%) were higher in non-fragmented spermatozoa. These findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.
Local renin angiotensin system and sperm DNA fragmentation_1
Local renin angiotensin system and sperm DNA fragmentation_2
Local renin angiotensin system and sperm DNA fragmentation_3
Local renin angiotensin system and sperm DNA fragmentation_4
  • High-resolution tumor marker detection based on microwave photonics demodulated dual wavelength fiber laser sensor
  • Jie Hu, Weihao Lin, Liyang Shao, Chenlong Xue, Fang Zhao, Dongrui Xiao, Yang Ran, Yue Meng, Panpan He, Zhiguang Yu, Jinna Chen, Perry Ping Shum
  • Opto-Electronic Advances
  • 2024-12-16
  • High performance laser induced plasma assisted ablation by GHz burst mode femtosecond pulses
  • Jingbo Yin, Yulong Zhao, Minghui Hong
  • Opto-Electronic Advances
  • 2024-12-16
  • Sequential harmonic spin–orbit angular momentum generation in nonlinear optical crystals
  • Yutao Tang, Zixian Hu, Junhong Deng, Kingfai Li, Guixin Li
  • Opto-Electronic Advances
  • 2024-12-16
  • Design, setup, and facilitation of the speckle structured illumination endoscopic system
  • Elizabeth Abraham, Zhaowei Liu
  • Opto-Electronic Science
  • 2024-12-13
  • Ultra-high-Q photonic crystal nanobeam cavity for etchless lithium niobate on insulator (LNOI) platform
  • Zhi Jiang, Cizhe Fang, Xu Ran, Yu Gao, Ruiqing Wang, Jianguo Wang, Danyang Yao, Xuetao Gan, Yan Liu, Yue Hao, Genquan Han
  • Opto-Electronic Advances
  • 2024-10-31
  • Advanced biological imaging techniques based on metasurfaces
  • Yongjae Jo, Hyemi Park, Hyeyoung Yoon, Inki Kim
  • Opto-Electronic Advances
  • 2024-10-31
  • Orthogonal matrix of polarization combinations: concept and application to multichannel holographic recording
  • Shujun Zheng, Jiaren Tan, Hongjie Liu, Xiao Lin, Yusuke Saita, Takanori Nomura, Xiaodi Tan
  • Opto-Electronic Advances
  • 2024-10-23
  • Streamlined photonic reservoir computer with augmented memory capabilities
  • Changdi Zhou, Yu Huang, Yigong Yang, Deyu Cai, Pei Zhou, Kuenyao Lau, Nianqiang Li, Xiaofeng Li
  • Opto-Electronic Advances
  • 2024-10-22
  • High-precision multi-focus laser sculpting of microstructured glass
  • Kang Xu, Peilin Huang, Lingyu Huang, Li Yao, Zongyao Li, Jiantao Chen, Li Zhang, Shaolin Xu
  • Opto-Electronic Advances
  • 2024-10-09
  • Multi-physical field null medium: new solutions for the simultaneous control of EM waves and heat flow
  • Sailing He, Ruili Zhang, Junbo Liang
  • Opto-Electronic Advances
  • 2024-09-30
  • Adaptive decentralized AI scheme for signal recognition of distributed sensor systems
  • Shixiong Zhang, Hao Li, Cunzheng Fan, Zhichao Zeng, Chao Xiong, Jie Wu, Zhijun Yan, Deming Liu, Qizhen Sun
  • Opto-Electronic Advances
  • 2024-09-29
  • Data-driven polarimetric approaches fuel computational imaging expansion
  • Sylvain Gigan
  • Opto-Electronic Advances
  • 2024-09-28



  • A narrative review of glaucoma screening from fundus images                                Electromagnetic bronchoscopy guided microwave ablation for early stage lung cancer presenting as ground glass nodule
    About
    |
    Contact
    |
    Copyright © PubCard